Essential fungal genetics / David Moore, Lily Ann Novak Frazer.

Format:

Book

Author/Creator:

Moore, D. (David), 1942-

Contributor:

Frazer, LilyAnn Novak, 1965-

Language:

English

Subjects (All):

Fungi--Genetics.

Fungi--genetics.

Genome, Fungal.

Medical Subjects:

Fungi--genetics.

Genome, Fungal.

Physical Description:

xi, 357 pages : illustrations ; 25 cm

Place of Publication:

New York : Springer, [2002]

Summary:

Challenging and attractive, comprehensive yet succinct, Essential Fungal Genetics was written to provide readers with the tools necessary to integrate fungal genetics into any number of general genetics, microbiology, mycology, and organismal biology courses by complementing the major graduate and undergraduate texts. Essential Fungal Genetics incorporates all aspects of eukaryote genetics appropriate to fungi, described here in an easily understood and remembered manner. The authors have taken an instructional "how-to" approach that will prove valuable for anyone wishing to start genetic analysis of any fungus. Rather than repeating "laboratory recipes," the authors strive to focus on the critical underlying concepts of fungal genetics, to maintain a readable style of presentation, and to provide a broad range of reference materials.

Contents:

Chapter 1 Why Study the Genomes of Fungi? 1

1.1 Origins 2

1.2 Diversity in the Kingdom Fungi: Chytridiomycota, Zygomycota, Ascomycota, and Basidiomycota 8

1.3 Fungi in Nature 14

1.4 Fungi in Technology 17

1.5 Fungi as Models 19

1.6 Genes to Genomics 21

Publications and Websites Worth a Visit 24

Historical Publications Worth Knowing About 25

Chapter 2 Genome Interactions 26

2.1 Fungal Lifestyles: Hyphal Fusions Are the Key to Advanced Hyphal Systems 28

2.2 Population Biology Aspects of Compatibility Systems 34

2.3 Compatibility and the Individualistic Mycelium 39

2.4 Nuclear Migration 44

2.5 Other Incompatibility Reactions 48

2.6 Structure and Function of Mating-Type Factors: Mating-Type Factors in Saccharomyces cerevisiae 51

2.7 Structure and Function of Mating-Type Factors: Mating-Type Factors of Neurospora 55

2.8 Structure and Function of Mating-Type Factors: Mating-Type Factors in Ustilago maydis 59

2.9 Structure and Function of Mating-Type Factors: Mating-Type Factors in Coprinus cinereus and Schizophyllum commune 62

2.10 Overview: Biology of Incompatibility Factors 67

Publications and Websites Worth a Visit 69

Historical Publications Worth Knowing About 70

Chapter 3 Wild Types and Mutants 71

3.1 Phenotypes and Genotypes: Variations and Mutations 72

3.2 Molecular Nature of Mutation 74

3.3 Natural Variation and Spontaneous Mutation 76

3.4 Generating Mutants: Mutagenesis 77

3.5 Generating Mutants: Irradiation 79

3.6 Generating Mutants: Chemical Mutagens 81

3.7 Types of Functional Mutant 83

3.8 Isolating Auxotrophic Mutants 84

3.9 Resistance Mutations 89

3.10 Reverse Mutation: From Auxotroph to Prototroph 90

3.11 Molecular Variants 91

Publications and Websites Worth a Visit 93

Historical Publications Worth Knowing About 94

Chapter 4 Segregation Genetics: The Indirect Approach 96

4.1 Complementing Mutants 97

4.2 Adenine Auxotrophs of Coprinus 98

4.3 Functional Allelism 104

4.4 Gene Segregation Depends on the Behavior of Chromosomes During Nuclear Division 106

4.5 Meiosis 107

4.6 Analyzing Gene Segregations from Random Spores 109

4.7 Use of x[superscript 2] Tables 111

4.8 Testing for Homogeneity 113

4.9 Detecting Linkage 118

Publications and Websites Worth a Visit 120

Historical Publications Worth Knowing About 121

Chapter 5 Recombination Analysis 122

5.1 Linkage Studies Make Maps 124

5.2 Multipoint Crosses 126

5.3 Rules of the Three-Point Crosses Game 129

5.4 A Three-Point Cross in Coprinus 130

5.5 Mapping Centromeres Using Gene Segregations in Tetrads and Eight-Spored Asci (Octads): Single Gene Segregations 132

5.6 Mapping Using Multiple Gene Segregations in Tetrads and Octads 135

5.7 Unordered Tetrads 140

5.8 Linkage Analysis to Linkage Map 145

5.9 Tetrad Segregations Leading to Secondary Homothallism 146

5.10 Gene Segregation During the Mitotic Division Cycle 147

5.11 Cytoplasmic Segregations: Mitochondria, Plasmids, Viruses, and Prions 154

Publications and Websites Worth a Visit 159

Historical Publications Worth Knowing About 160

Chapter 6 Mechanisms of Recombination 161

6.1 Gene Conversion 162

6.2 Hybrid- or Heteroduplex-DNA 164

6.3 A Basic Mechanism for Recombination 166

6.4 Correction of Base Mismatches in Heteroduplex-DNA Generates Aberrant Segregation Ratios 170

6.5 Modifying the Basic Recombination Model 172

6.6 Models and the Real World 177

Publications and Websites Worth a Visit 182

Historical Publications Worth Knowing About 183

Chapter 7 The Physical Genotype 184

7.1 Molecular Markers 186

7.2 DNA Polymorphisms 187

7.3 Restriction Fragment Length Polymorphisms 188

7.4 Polymerase Chain Reaction 192

7.5 PCR Primers: AP-PCR and RAPD 193

7.6 Single-Strand Conformation Polymorphisms 195

7.7 DNA Fingerprinting 196

7.8 Microsatellites 199

7.9 Minisatellites 200

7.10 Transposable Elements 201

7.11 Genes and Spacers 204

7.12 Electrophoretic Karyotypes 205

Publications and Websites Worth a Visit 207

Historical Publications Worth Knowing About 209

Chapter 8 Genes to Genomics: Mapping the Fungal Genome 211

8.1 Genes and Maps: The Story So Far 212

8.2 Physical Maps 215

8.3 Restriction Mapping: A Real-Life Example 216

8.4 Optical Mapping 223

8.5 DNA Cloning: Plasmids, Cosmids, BACs, and YACs 225

8.6 Chain Termination Sequencing 227

8.7 The First Complete Eukaryotic Genome Sequence: Saccharomyces cerevisiae 233

8.8 Comparisons Between Genomes 236

Publications and Websites Worth a Visit 240

Historical Publications Worth Knowing About 243

Chapter 9 Systematics, Phylogeny, and Evolution 245

9.1 Phylogenetics: Inferring Evolutionary Development 246

9.2 The Molecule Is the Message 248

9.3 Inferring Relationships 251

9.4 Making Trees Make Sense 256

9.5 Horizontal Transfer of Genetic Information 261

9.6 Genes in Populations 262

9.7 Genes in Fungal Populations 266

9.8 Genetic Variation in Hosts and Pathogens 270

9.9 Evolution in Captivity: Natural and Artificial Selection 273

9.10 Mycotechnology 275

Publications and Websites Worth a Visit 279

Historical Publications Worth Knowing About 281

Chapter 10 The Genetics of Fungal Differentiation and Morphogenesis 282

10.1 Differentiation and Morphogenesis 284

10.2 Genetic Approaches for Analyzing Gene Regulation 286

10.3 Regulating Gene Expression: DNA-Binding Proteins 288

10.4 Regulating Gene Expression: Chromatin Remodeling 290

10.5 Regulating Gene Expression: Transcription 292

10.6 Galactose Utilization in Yeast: The Epitome of Eukaryote Regulation 295

10.7 Regulating Gene Expression: Repression and Silencing 298

10.8 Regulating Gene Expression: High-Level Control Mechanisms, DNA Modification, and Epigenetics 300

10.9 Posttranscriptional Regulation: Spliceosomes, Proteasomes, and Protein Networks 305

10.10 Shape, Form, and Differential Gene Expression 310

10.11 Yeast-Mycelial Dimorphism 311

10.12 Conidiation: Translational Triggering and Feedback Fixation 315

10.13 Sexual Reproductive Structures in Ascomycetes and Basidiomycetes 321

10.14 Genetic Control of Morphogenesis of Fungal Fruit Bodies 332.

Notes:

Includes bibliographical references and index.

ISBN:

0387953671

OCLC:

48177277