Culture of epithelial cells / editors, R. Ian Freshney and Mary G. Freshney.

Format:

Book

Contributor:

Freshney, R. Ian.

Freshney, Mary G.

Series:

Culture of specialized cells

Language:

English

Subjects (All):

Epithelial cells.

Cell culture.

Epithelium--Cultures and culture media.

Epithelium.

Epithelial Cells.

Cell Differentiation.

Cells, Cultured.

Cytological Techniques.

Medical Subjects:

Epithelial Cells.

Cell Differentiation.

Cells, Cultured.

Cytological Techniques.

Physical Description:

xv, 461 pages : illustrations ; 26 cm.

Edition:

Second edition.

Place of Publication:

New York : Wiley-Liss, [2002]

Summary:

Culture of Specialized Cells CULTURE OF EPITHELIAL CELLS, Second Edition Edited by R. Ian Freshney and Mary G. Freshney

" It is now the age of the specialized cell in culture, " R. Ian Freshney wrote in the preface to the first edition of Culture of Epithelial Cells. Realizing the inadequacy of studying all types of cells at random in a culture, it became imperative for researchers to learn how unique cells perform under a wide variety of conditions. Now the Second Edition of Culture of Epithelial Cells- - devoted to those cells that line the body, internally and externally, protecting surfaces and secreting or absorbing material- - advances this ethic, providing completely revised, detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia.

Each chapter of the Second Edition is devoted to a different type of epithelium- - epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. R. Ian Freshney also contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form with additional protocols. Other features of the Second Edition of Culture of Epithelial Cells include: Proven methods refined by the authors, all well-established researchers Alternate techniques coupled with critical comparative analyses Logical anatomical organization by tissue type Stepwise culture protocols conveniently highlighted New chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium In-depth analysis of cell-cellinteraction in skin

Displaying the clear, easy-to-follow style that has become a trademark of the Culture of Specialized Cells series, the Second Edition of Culture of Epithelial Cells remains an essential resource for researchers in cell and developmental biology, cancer biology, tissue engineering, biochemistry, and genetics, where the work requires the cultivation or manipulation of cells from the epithelium.

Contents:

Chapter 2. Cell Interaction and Epithelial Differentiation / Nicole Maas-Szabowski, Hans-Jurgen Stark, Norbert E. Fusenig 31

Chapter 3. The Epidermis / E. Kenneth Parkinson, W. Andrew Yeudall 65

Chapter 4. Culture of Human Mammary Epithelial Cells / Martha R. Stampfer, Paul Yaswen, Joyce Taylor-Papadimitriou 95

Chapter 5. Culture of Human Cervical Epithelial Cells / Margaret A. Stanley 137

Chapter 6. Human Prostatic Epithelial Cells / Donna M. Peehl 171

Chapter 7. Human Oral Epithelium / Roland G. Grafstrom 195

Chapter 8. Normal Human Bronchial Epithelial Cell Culture / John Wise, John F. Lechner 257

Chapter 9. Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells / Leland G. Dobbs, Robert F. Gonzalez 277

Chapter 10. Isolation and Culture of Intestinal Epithelial Cells / Catherine Booth, Julie A. O'Shea 303

Chapter 11. Isolation and Culture of Animal and Human Hepatocytes / Christiane Guguen-Guillouzo 337

Chapter 12. Culture of Human Urothelium / Jennifer Southgate, John R. W. Masters, Ludwik K. Trejdosiewicz 381

Chapter 13. Other Epithelial Cells / R. Ian Freshney 401.

Notes:

Includes bibliographical references and index.

ISBN:

0471401218

OCLC:

47522467