Enzyme technologies for pharmaceutical and biotechnological applications / edited by Herbert A. Kirst, Wu-Kuang Yeh, Milton J. Zmijewski, Jr.

Format:

Book

Contributor:

Kirst, Herbert A.

Yeh, Wu-Kuang, 1942-

Zmijewski, Milton J., 1948-

Rosengarten Family Fund.

Language:

English

Subjects (All):

Enzymes--Biotechnology.

Enzymes.

Enzymes--Industrial applications.

Pharmaceutical biotechnology.

Physical Description:

xv, 611 pages : illustrations ; 24 cm

Place of Publication:

New York : Marcel Dekker, [2001]

Contents:

I. Biosynthesis

1. [delta]-(L-[alpha]-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase as a Model Tripeptide Synthetase / Hans von Dohren, Wibke Kallow, Mary Anne Tavanlar, Torsten Schwecke, Ralf Dieckmann, Volker Uhlmann 1

2. Metabolic Engineering for Cephalosporin C Yield Improvement and Production of Intermediates / Joe E. Dotzlaf, Steven W. Queener, Wu-Kuang Yeh 39

3. Bioconversion of Penicillins to Cephalosporins / Arnold L. Demain, Jose L. Adrio, Jacqueline M. Piret 61

4. Direct Fermentative Production of Acyltylosins by Genetically Engineered Strains of Streptomyces fradiae / Akira Arisawa, Hiroshi Tsunekawa 89

5. Engineering Streptomyces avermitilis for the Production of Novel Avermectins: Mutant Design and Titer Improvement / Claudio D. Denoya, Kim J. Stutzman-Engwall, Hamish A. I. McArthur 113

II. Biocatalysis

6. Biocatalytic Syntheses of Chiral Intermediates for Antihypertensive Drugs / Ramesh N. Patel 137

7. Cloning, Structure, and Activity of Ketone Reductases from Baker's Yeast / Jon D. Stewart, Sonia Rodriguez, Margaret M. Kayser 175

8. Cross-Linked Enzyme Crystals: Biocatalysts for the Organic Chemist / Michael D. Grim 209

9. Enzymatic Deacylation of Echinocandins and Related Antifungal Agents / Andrew R. Cockshott, Adam J. Kreuzman, Wu-Kuang Yeh 227

III. Screening/Optimization

10. Roles of Enzymes in Antibacterial Drug Discovery / Siddhartha Roychoudhury 245

11. Penicillin-Binding Proteins as Antimicrobial Targets: Expression, Purification, and Assay Technologies / Genshi Zhao, Timothy I. Meier, Wu-Kuang Yeh 263

12. Development of a High-Throughput Screen for Streptococcus pneumoniae UDP-N-Acetylmuramoyl-Alanine: D-Glutamate Ligase (MurD) for the Identification of MurD Inhibitors / Michele C. Smith, James A. Cook, Gary M. Birch, Stephen A. Hitchcock, Robert B. Peery, Joann Hoskins, Paul L. Skatrud, Raymond C. Yao, Karen L. Cox 289

13. Purification and Assay Development for Human Rhinovirus Proteases / Q. May Wang, Robert B. Johnson 307

14. Screening for Parasiticides Using Recombinant Microorganisms / Timothy G. Geary 323

15. Screening for Inhibitors of Lipid Metabolism / Hiroshi Tomoda, Satoshi Omura 343

16. Design and Development of a Selective Assay System for the Phospholipase A[subscript 2] Superfamily / Hsiu-Chiung Yang, Marian Mosior, Edward A. Dennis 379

IV. Emerging Technologies

17. Understanding and Exploiting Bacterial Polyketide Synthases / Robert McDaniel, Chaitan Khosla 397

18. Polyketide Synthases: Analysis and Use in Synthesis / Kira J. Weissman, James Staunton 427

19. Enzymatic Synthesis of Fungal N-Methylated Cyclopeptides and Depsipeptides / Mirko Glinski, Till Hornbogen, Rainer Zocher 471

20. New Strategies for Target Identification, Validation, and Use of Enzymes in High-Throughput Screening / Joaquim Trias, Zhengyu Yuan 499

21. Use of Genomics for Enzyme-Based Drug Discovery / Molly B. Schmid 515

22. Assigning Precise Function to Genes / Ridong Chen 537

23. Redesigning Binding and Catalytic Specificities of Enzymes / Ridong Chen 555

24. Proteomics: Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods / Srinivasan Krishnan, John E. Hale, Gerald W. Becker 575.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Rosengarten Family Fund.

ISBN:

0824705491

OCLC:

46791061