1 option
Epithelial cell culture protocols / edited by Clare Wise.
Holman Biotech Commons QH506 .M45 v.188 2002
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; 188.
- Methods in molecular biology ; 188
- Language:
- English
- Subjects (All):
- Epithelial cells--Laboratory manuals.
- Epithelial cells.
- Epithelium--Cultures and culture media--Laboratory manuals.
- Epithelium.
- Cell culture--Laboratory manuals.
- Cell culture.
- Epithelial Cells.
- Epithelium--Cultures and culture media.
- Cell Culture Techniques.
- Cells, Cultured.
- Medical Subjects:
- Epithelial Cells.
- Epithelium.
- Cell Culture Techniques.
- Cells, Cultured.
- Genre:
- Laboratory manuals.
- Physical Description:
- xiv, 405 pages : illustrations ; 24 cm.
- Place of Publication:
- Totowa, NJ : Humana Press, [2002]
- Summary:
- Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.
- Contents:
- 1 Human Lens Epithelial Cell Culture / Nobuhiro Ibaraki 1
- 2 Human Airway Epithelial Cell Culture / Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, Hidetada Sasaki 7
- 3 Rat Gastric Mucosal Epithelial Cell Culture / Yoshitaka Konda, Tsutomu Chiba 17
- 4 Thymic Epithelial Cell Culture / Carsten Ropke 27
- 5 Bile Duct Epithelial Cell Culture / Alphonse E. Sirica 37
- 6 Liver Epithelial Cell Culture / Rolf Gebhardt 53
- 7 Pulmonary Epithelial Cell Culture / Ben Forbes 65
- 8 Prostate Epithelial Cell Isolation and Culture / David L. Hudson 77
- 9 A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier / Albert Guidry, Celia O'Brien 85
- 10 The Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells / Wei Zheng, Qiuqu Zhao 99
- 11 An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures / Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, Michael J. Welsh 115
- 12 Primary Mouse Keratinocyte Culture / Kairbaan Hodivala-Dilke 139
- 13 Analyzing Apoptosis in Cultured Epithelial Cells / Andrew P. Gilmore, Charles H. Streuli 145
- 14 Keratins as Markers of Epithelial Cells / David L. Hudson 157
- 15 Epithelial Cell Integrins / Joachim Rychly 169
- 16 Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium / Sebastian Zellmer, Dieter Reissig 179
- 17 Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces / Norio Kumagai 185
- 18 Transplantation of Cultured Epithelial Cells / Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, Ken-Ichiro Hata 197
- 19 An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation / Tohru Masui 213
- 20 Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract / Jie Shen, Vincent H. L. Lee 217
- 21 Applications of Epithelial Cell Culture in Studies of Drug Transport / Staffan Tavelin, Johan Grasjo, Jan Taipalensuu, Goran Ocklind, Per Artursson 233
- 22 X-Ray Microanalysis of Epithelial Cells in Culture / Godfried M. Roomans 273
- 23 ENU Mutagenesis of Rat Mammary Epithelial Cells / George Stoica 291
- 24 ENU-Induced Ovarian Cancer / George Stoica 305
- 25 Measurement of Membrane Capacitance in Epithelial Monolayers / Carol A. Bertrand, Ulrich Hopfer 315
- 26 Assessing Epithelial Cell Confluence by Spectroscopy / Simon A. Lewis 329
- 27 Co-Cultivation of Liver Epithelial Cells with Hepatocytes / Rolf Gebhardt 337
- 28 Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium / Wee Soo Shin, Chieko Hemmi, Teruhiko Toyo-oka 347
- 29 Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability / Derek M. McKay, Mary H. Perdue 359
- 30 Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals / Carin Sahlberg, Tuija Mustonen, Irma Thesleff 373
- 31 Bacterial Interactions with Host Epithelium In Vitro / Nicola Jones, Mary H. Perdue, Philip M. Sherman, Derek M. McKay 383.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 0896038939
- OCLC:
- 47690090
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.