Practical immunology / Frank C. Hay, Olwyn M.R. Westwood ; with the assistance of Paul N. Nelson.

Format:

Book

Author/Creator:

Hay, Frank C.

Contributor:

Westwood, Olwyn M. R.

Nelson, Paul N.

Hudson, Leslie.

Language:

English

Subjects (All):

Immunology--Laboratory manuals.

Immunology.

Immunologic Techniques.

Allergy and Immunology.

Medical Subjects:

Immunologic Techniques.

Allergy and Immunology.

Genre:

Laboratory manuals.

Physical Description:

vii, 400 pages : illustrations ; 25 cm

Edition:

Fourth edition.

Place of Publication:

Oxford ; Malden, MA : Blackwell Science, 2001.

Summary:

Practical Immunology is an introductory text aimed at students and researchers who need a comprehensive overview of the methodology of immunology. Now in its fourth edition, Practical Immunology will appeal to students of immunology, graduate students embarking on bench science, and specialized immunologists who need to use a technique outside their sphere of expertise.

The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice. Practical Immunology gives straightforward descriptions of the more commonly used techniques that are often covered, but not always clearly described, in the research literature. It explains how to perform these basic methodologies that form the anatomy of experimental immunology, and includes tips for improving the techniques, troubleshooting and other helpful suggestions. Where possible, the methods are explained in a way to ensure that the purchase of specialist kits is avoidable. For those who are a little out of practice on their basic immunology, the book also gives some background information, making it a highly useful 'one stop shop'.

This latest edition has been fully revised to include basic techniques found in the appropriate chapters, with revised sections on cytokines, clinical immunology, new techniques in epitope mapping and the study of cell adhesion molecules.

Contents:

1 Isolation and structure of immunoglobulins 1

2 Monoclonal antibodies: production, purification and enzymatic fragmentation 40

3 Antibody interactions with antigens 71

4 Antibodies as probes 115

5 Immunoassay 163

6 Isolation of cells 179

7 Phagocytosis, complement and antibody-dependent cytotoxicity 203

8 Lymphocyte structure 228

9 Lymphocyte function 261

10 The cytokines 301

11 Immunological manipulations in vivo 326

Appendix A Buffers and media 346

Appendix B Basic techniques and useful data 353

Appendix C Equipment and manufacturers index 377.

Notes:

Rev. ed. of: Practical immunology / Leslie Hudson, Frank C. Hay. 3rd ed. 1989.

Includes bibliographical references and index.

Related Work:

Hudson, Leslie. Practical immunology.

ISBN:

0865429618

OCLC:

249344737