In vitro mutagenesis protocols / edited by Jeff Braman.

Format:

Book

Contributor:

Braman, Jeff.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 182.

Methods in molecular biology ; 182

Language:

English

Subjects (All):

Mutagenesis--Methodology.

Mutagenesis.

Mutagenesis, Site-Directed.

Mutagenesis, Insertional--methods.

Polymerase Chain Reaction.

Medical Subjects:

Mutagenesis, Site-Directed.

Mutagenesis, Insertional--methods.

Polymerase Chain Reaction.

Genre:

Laboratory manuals.

Physical Description:

xvi, 287 pages : illustrations ; 24 cm.

Edition:

Second edition.

Place of Publication:

Totowa, N.J. : Humana Press, [2002]

Summary:

Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

Notes:

Includes bibliographical references and index.

ISBN:

0896039102

OCLC:

47100807