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Antibody phage display : methods and protocols / edited by Philippa M. O'Brien and Robert Aitken.
Holman Biotech Commons QH506 .M45 v.178 2002
Available
Holman Biotech Commons QH506 .M45 v.178 2002
Available
Levy Dental Medicine Library - Stacks QR186.85 .A585 2002
Available
- Format:
- Book
- Series:
- Methods in molecular biology (Clifton, N.J.) ; v. 178.
- Methods in molecular biology ; v. 178
- Language:
- English
- Subjects (All):
- Monoclonal antibodies--Research--Methodology.
- Monoclonal antibodies.
- Bacteriophages.
- Antibodies, Monoclonal.
- Monoclonal antibodies--Research.
- Methodology.
- Medical Subjects:
- Antibodies, Monoclonal.
- Bacteriophages.
- Physical Description:
- xiii, 401 pages : illustrations ; 24 cm.
- Place of Publication:
- Totowa, N.J. : Humana Press, 2002.
- Summary:
- This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.
- Contents:
- 1 Overview of Antibody Phage-Display Technology and Its Applications / Hennie R. Hoogenboom 1
- 2 Standard Protocols for the Construction of Fab Libraries / Michelle A. Clark 39
- 3 Standard Protocols for the Construction of scFv Libraries / Simon Lennard 59
- 4 Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice / Philippa M. O'Brien, Robert Aitken 73
- 5 Construction of Large Naive Fab Libraries / Hans J. W. de Haard 87
- 6 Construction of Polyclonal Antibody Libraries Using Phage Display / Jacqueline Sharon, Seshi R. Sompuram, Chiou-Ying Yang, Brent R. Williams, Stefanie Sarantopoulos 101
- 7 Antigen-Driven Stimulation of B-Lymphocytes In Vitro / Zhiwei Hu 113
- 8 The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion / Ruud M. T. de Wildt, Rene M. A. Hoet 121
- 9 Panning of Antibody Phage-Display Libraries: Standard Protocols / David W. J. Coomber 133
- 10 Selection of Antibodies Against Biotinylated Antigens / Patrick Chames, Hennie R. Hoogenboom, Paula Henderikx 147
- 11 Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries / Keith A. Charlton, Andrew J. Porter 159
- 12 Blocking Immunodominant Epitopes by Competitive Deselection / Roberto Burioni 173
- 13 Rescue of a Broader Range of Antibody Specificities Using an Epitope-Masking Strategy / Henrik J. Ditzel 179
- 14 Screening of Phage-Expressed Antibody Libraries by Capture Lift / Jeffry D. Watkins 187
- 15 Antibody-Guided Selection Using Capture-Sandwich ELISA / Kunihiko Itoh, Toshio Suzuki 195
- 16 Proximity-Guided (ProxiMol) Antibody Selection / Jane K. Osbourn 201
- 17 Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies / Mariangela Figini, Silvana Canevari 207
- 18 Selecting Antibodies to Cell-Surface Antigens Using Magnetic Sorting Techniques / Don L. Siegel 219
- 19 Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs / Elvyra J. Noronha, Xinhui Wang, Soldano Ferrone 227
- 20 Subtractive Isolation of Single-Chain Antibodies Using Tissue Fragments / Katarina Radosevic, Willem van Ewijk 235
- 21 Selection of Antibodies Based on Antibody Kinetic Binding Properties / Ann-Christin Malmborg, Nina Nilsson, Mats Ohlin 245
- 22 Selection of Functional Antibodies on the Basis of Valency / Manuela Zaccolo 255
- 23 Two-Step Strategy for Alteration of Immunoglobulin Specificity by In Vitro Mutagenesis / Yoshitaka Iba, Chie Miyazaki, Yoshikazu Kurosawa 259
- 24 Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement / Partha S. Chowdhury 269
- 25 Error-Prone Polymerase Chain Reaction for Modification of scFvs / Pierre Martineau 287
- 26 Use of Escherichia coli Mutator Cells to Mature Antibodies / Robert A. Irving, Gregory Coia, Anna Raicevic, Peter J. Hudson 295
- 27 Chain Shuffling to Modify Properties of Recombinant Immunoglobulins / Johan Lantto, Pernilla Jirholt, Yvelise Barrios, Mats Ohlin 303
- 28 Generation of Bispecific and Tandem Diabodies / Sergey M. Kipriyanov 317
- 29 High-Level Periplasmic Expression and Purification of scFvs / Sergey M. Kipriyanov 333
- 30 Periplasmic Expression and Purification of Recombinant Fabs / Robert L. Raffai 343
- 31 Expression of Antibody Fragments in Pichia pastoris / Philipp Holliger 349
- 32 Expression of V[subscript HH] Antibody Fragments in Saccharomyces cerevisiae / J. Marcel van der Vaart 359
- 33 Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments / Pascale A. Cohen 367
- 34 Expression of scFvs and scFv Fusion Proteins in Eukaryotic Cells / Michelle de Graaf, Ida H. van der Meulen-Muileman, Herbert M. Pinedo, Hidde J. Haisma 379
- 35 Expression of Antibody Fab Fragments and Whole Immunoglobulin in Mammalian Cells / Pietro P. Sanna 389.
- Notes:
- Includes bibliographical references and index.
- ISBN:
- 0896039064
- 0896037118
- OCLC:
- 47894259
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