Grauzone and completion of meiosis during drosophila oogenesis / by Bin Chen.

Format:

Book

Author/Creator:

Chen, Bin, 1970-

Language:

English

Subjects (All):

Zinc-finger proteins.

Meiosis.

Drosophila.

Oogenesis.

Meiosis--genetics.

Drosophila--cytology.

Oogenesis--physiology.

Zinc Fingers--genetics.

Medical Subjects:

Meiosis--genetics.

Drosophila--cytology.

Oogenesis--physiology.

Zinc Fingers--genetics.

Physical Description:

xx, 80 pages : illustrations ; 24 cm

Place of Publication:

Boston : Kluwer Academic Publishers, [2001]

Summary:

Grauzone and Completion of Meiosis During Drosophila Oogenesis describes the work behind a major, award winning discovery: the establishment of a new pathway that specifically regulates the female meiosis, a process essential for sexual reproduction. This book chronicles a new gene mapping method and the cloning and documentation of several types of genes that were proven to have significant influence on the cell cycle. It is of interest to anyone doing work with fruit flies, both graduate students and principal investigators.

Contents:

1. Female Meiotic Cell Cycle 1

2. Genesis and Meiosis in Drosophila Females 4

3. Cell Cycle Regulation of Drosophila Female Meiosis 5

4. Mutations in Grauzone (Grau) and Cortex (Cort) Block Progression of Drosophila Female Meiosis and Cause Other Defects 6

5. Goals and Significance of This Dissertation Research 7

Mapping of Drosophila Mutations Using Site-Specific Male Recombination 11

2. Materials and Methods 13

2.1 Drosophila stocks 13

2.2 Complementation tests of grau mutations with chromosomal deficiencies and P element insertion lines 15

2.3 Generating recombinants by P-element-induced male recombination 15

2.4 Fertility tests of P element induced male recombinants 16

3. Results 17

3.1 Scheme for mapping Drosophila mutations using P element induced male recombination 17

3.2 Mapping the grau gene 19

3.3 Mapping the cort gene 22

Completion of Meiosis in Drosophila Oocytes Requires Transcriptional Control by Grauzone, A New Zinc Finger Protein 31

2. Experimental Procedures 33

2.1 Fly stocks and mapping 33

2.2 Genetic test between grau and cort 34

2.3 P-element-mediated transformation and rescue 35

2.4 Molecular techniques 35

2.5 Grau-GFP construct 35

2.6 Microscopy 36

2.7 Construction and purification of GST-Grau fusion protein 37

2.8 Gel shift assay 37

3. Results 38

3.1 Isolation of the grau gene 38

3.2 The Grau protein 42

3.3 Nature of the grau mutant alleles 44

3.4 Expression of the grau transcript during development 46

3.5 The Grau-GFP fusion protein is localized to the nucleus of both nurse cells and follicle cells 46

3.6 Interaction between grau and cort 47

3.7 Grau protein binds to the cort promoter 51

4.1 Mutations in the grau alleles affect the coding region of the protein 53

4.2 Regulation of Grau function during oogenesis and at later developmental stages 54

4.3 Control of meiotic divisions 55

4.4 Translational control, meiosis, and early development 57

1. Drosophila as a Model to Study Regulation of Female Meiotic Cell Cycle 61

2. Grau and Cort are Two Members of a New Pathway that Regulates the Meiotic Cell Cycle Machinery in Drosophila Females 62

3. Male Recombination Mapping is a General Method to Map Drosophila Autosomal Mutations 66.

Notes:

Includes bibliographical references (pages [69]-76) and index.

ISBN:

0792373634

OCLC:

46565988