Interleukin protocols / edited by Luke A. J. O'Neill and Andrew Bowie.

Format:

Book

Contributor:

O'Neill, Luke A. J.

Bowie, Andrew.

Series:

Methods in molecular medicine ; 60.

Methods in molecular medicine ; 60

Language:

English

Subjects (All):

Interleukins--Laboratory manuals.

Interleukins.

Medical Subjects:

Interleukins.

Genre:

Laboratory manuals.

Physical Description:

xv, 443 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, NJ : Humana Press, [2001]

Summary:

Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

Contents:

Part I General Methods for Assaying Interleukin Protein and mRNA 1

1 ELISAs and Interleukin Research / Catherine Greene 3

2 ELISPOT Technique for Assaying Interleukins / Almuth Christine Hauer, Mona Bajaj-Elliott 17

3 Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay) / Yoshihiro Okamoto, Mikio Nishida 29

4 Assaying Interleukins Intracellularly by FACS / Liam O'Mahony, Maurice O'Donoghue 41

5 Basic RT-PCR for Measurement of Cytokine Expression / Mona Bajaj-Elliott, Almuth Christine Hauer 49

6 Quantitative PCR for Measurement of Cytokine Expression / Ellen M. Nilsen, Finn-Eirik Johansen, Per Brandtzaeg 59

7 Single Cell RT-PCR for the Detection of Cytokine mRNA / Moshe Ligumsky, Hovav Nechushtan, Ehud Razin 77

8 Quantitation of Cytokine mRNA by Flash-Type Bioluminescence / Jeffrey K. Actor 83

9 Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method / Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, Johanne Martel-Pelletier 99

10 Quantification of Mouse IL-6 and TNF-[alpha] mRNA Using Xplore Assays / Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson, Mark D. Moody 111

11 Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay / Zheng-Ming Wang, Roman Dziarski 125

12 Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography / Terry M. Phillips, Benjamin F. Dickens 145

13 Detection of Cytokines by Immunohistochemistry / Mahmoud Huleihel 157

14 Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement / Nikolai Petrovsky 163

Part II Illustration of Methodologies Using Individual Interleukins 175

15 Assaying Interleukin-8 / Paul N. Moynagh 177

16 Measurement of Interleukin-2 by Bioassay / Lyanne Weston 185

17 Assaying Interleukin-15 / Amos Douvdevani, Michal Weiler, Eli Lewis 193

Part III Assaying Interleukins in Particular Pathologies 205

18 Assaying Interleukin-6 in Breast Cancer / Judith Harmey, Amanda Haverty, Deirdre Foley, David Bouchier-Hayes 207

19 Cytokines, Stress, and Depressive Illness / Hymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, Jenna Griffiths 217

20 Measurement of Interleukins in Cutaneous Disorders: Competitive RT-PCR for Determination of IL-10 mRNA Expression in Psoriasis / Khusru Asadullah, Antie Haeussler-Quade, Wolf Dietrich Doecke, Wolfram Sterry, Hans-Dieter Volk 239

21 Interleukins in Graves' Disease / Jan Komorowski 251

22 Evaluation of Cytokines in Migraine Patients by ELISA / Irene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, Vincenzo Centonze 263

23 Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction / Radek Pudil 273

Part IV Assaying Interleukins in Particular Biological Fluids 283

24 Measurement of Cytokines in Peritoneal Fluids / Reijo Punnonen, Juha Punnonen 285

25 Measurement of Cytokines in Seminal Plasma / Marek Glezerman, Eitan Lunenfeld, Mahmoud Huleihel 293

26 Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD / Peter G. Gibson, Jodie L. Simpson 305

27 Cytokines in Blister Fluids / Franco Ameglio, Luciano D'Auria 313

28 Measurement of Cytokines in Synovial Fluid / Fabrizio De Benedetti 327

29 Measurement of Cytokines in Cerebrospinal Fluid / Toshiyuki Yokoyama 337

30 Detection of Cytokines in Tears / Archana Thakur, Mark D. P. Willcox 345

Part V Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the Use of cDNA Arrays 355

31 Measurement of Phosphoinositide 3-Kinase Activation by Interleukins / Stephen G. Ward 357

32 Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis / Paul Brennan, Veronica Athie-Morales 369

33 Applications for Green Fluorescent Protein in Cell Signaling / Paul Brennan, Emmanuelle Astoul 385

34 Use of Pichia pastoris for Production of Recombinant Cytokines / Kevin P. Murphy, Csaba Pazmany, Mark D. Moody 393

35 Analysis of Single Nucleotide Polymorphisms in the Interleukin-1B Gene Using 5' Nuclease Assays / Emad M. El-Omar 409

36 Measurement of Cytokine and Chemokine Gene Expression Patterns Using cDNA Array / Zheng-Ming Wang, Roman Dziarski 419.

Notes:

Includes bibliographical references and index.

ISBN:

089603738X

OCLC:

45890344