Experiments in applied microbiology / Samuel Singer.

Format:

Book

Author/Creator:

Singer, Samuel.

Language:

English

Subjects (All):

Microbiology--Experiments.

Microbiology.

Physical Description:

ix, 416 pages : illustrations ; 28 cm

Place of Publication:

San Diego : Academic Press, [2001]

Summary:

This is a lab manual intended for use in undergraduate and some graduate courses. The experiments are designed to illustrate the fundamental principles of bacteriology, microbial ecology, and bacterial physiology. While other books in this area deal primarily with DNA techniques and related methodology for constructing useful bacterial strains. This text shows how to use these strains to produce and/or modify a product or process.

Contents:

Section I Diversity

1. Physiological Diversity 3

2. Differentiation of Bacillus Using Biochemical Characteristics 24

3. Other Isolation Techniques: Mosquito Larvae, Thermophiles, Submerged Slide 51

4. Isolation of Morphological Group I, II, and III Strains of Bacillus 68

5. Winogradsky Column: Examination of Succession of Microbial Populations in Soil 86

6. Bacterial Nutrition of Photolithotrophs, Photoorganotrophs, Chemolithotrophs, and Chemoorganotrophs 97

7. Effect of Detritus on the Growth of Aquatic Bacteria 145

Section II Fermentation

8. The Standardized Culture 159

9. Shake-Flask Baseline Fermentation Experiments: Role of Media 171

10. Shake-Flask Baseline Fermentation Experiments: Role of Physical Factors 193

11. Shake-Flask Baseline Fermentation Experiments: Role of Culture and Culture Selection 204

12. Small Bioreactor Fermentation Experiments: Translation from a Shaken to a Stirred Vessel 221

13. Effect of Enzymes on In-Cycle Examination of Larvicidal Toxins during Fermentation Process 237

14. Ureolytic Activity in Bacteria 255

Section III Bacteria-Invertebrate Interface

15. Bacteria and Culex quinquefasciatus Larval Gut 269

16. Galleria mellonella Larvae as Toxin Indicators 289

17. Early Feeding Effects of Bacterial Larvicides on Culex quinquefasciatus Larvae 304

18. Larvicidal Effects on Field-Collected Black Fly Larvae (Simulium) 324

19. Maintenance (Rearing) and Bioassay Using the Nematodes Caenorhabditis elegans and Heterodera glycines 342

20. Maintenance (Rearing) and Bioassay Using the Cigarette Beetle Lasioderma serricorne and the Indian Meal Moth Plodia interpunctella 362

21. Maintenance (Rearing) and Bioassay Using the Zebra Mussel (Dreissena polymorpha) and the Snail (Biomphalaria glabrata) 376.

Notes:

Includes bibliographical references and index.

ISBN:

0126459606

OCLC:

47263217