Atherosclerosis : experimental methods and protocols / edited by Angela F. Drew.

Format:

Book

Contributor:

Drew, Angela F.

Series:

Methods in molecular medicine ; 52.

Methods in molecular medicine ; 52

Language:

English

Subjects (All):

Atherosclerosis.

Clinical Protocols.

Research Design.

Medical Subjects:

Atherosclerosis.

Clinical Protocols.

Research Design.

Physical Description:

xi, 247 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2001.

Summary:

In Atherosclerosis: Experimental Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Comprehensive and richly detailed, Atherosclerosis: Experimental Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.

Contents:

1 Animal Models of Diet-Induced Atherosclerosis / Angela F. Drew 1

2 Mechanical Injury Models: Balloon Catheter Injury to Rat Common Carotid Artery / Rodney J. Dilley 7

3 Genetically Manipulated Models of Atherosclerosis in Mice / Qing Xiao 15

4 Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation / Kishor M. Wasan, Shawn M. Cassidy, Allison L. Kennedy, Kathy D. Peteherych 27

5 Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol / Joan A. Higgins, John M. Graham, Ian G. Davies 37

6 Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Lodixanol / John M. Graham, Bruce A. Griffin, Ian G. Davies, Joan A. Higgins 51

7 Detecting Cholesteryl Ester Transfer Protein in Plasma / Andreas Ritsch, Josef R. Patsch 61

8 Determination of Plasma Homocysteine / Roberto Accinni, Oberdan Parodi 77

9 Assay for Serum Glycated Lipoproteins / Akira Tanaka 105

10 Lipoprotein(a) Quantitation / Michael Bender, Werner Gross 113

11 Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels / Eleni Papakonstantinou, Michael Roth, George Karakiulakis 123

12 Culture of Human Smooth Muscle Cells / Marisa A. Gallicchio 137

13 Culture of Human Endothelial Cells / Marisa A. Gallicchio 147

14 Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells / George Karakiulakis, Eleni Papakonstantinou, Michael Roth 163

15 Collection and Processing of Arterial Specimens for Histological Analysis / Angela F. Drew 171

16 Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo / Hannu I. Manninen 177

17 Identification of Cell Types and Quantification of Lesion Composition / Rodney J. Dilley 187

18 Nonradioactive In Situ Hybridization in Atherosclerotic Tissue / Jim Apostolopoulos 195

19 Evaluation of Angiogenesis / Marcy Silver, Jeffrey M. Isner 207

20 Evaluation of Cell Proliferation in Human Atherosclerotic Lesions / Elena R. Andreeva, Alexander N. Orekhov 213

21 Gene Transfer to the Vasculature: Historical Perspective and Implications for Future Research Objectives / Sarah J. George, Andrew H. Baker 219

22 A Pig Model of Vein Graft Disease: Applications for Potential Gene Therapies / Clinton T. Lloyd, Sarah J. George, Gianni D. Angelini, Andrew C. Newby, Andrew H. Baker 233.

Notes:

Includes bibliographical references and index.

ISBN:

0896037517

OCLC:

43801051