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In situ hybridization in light microscopy / Gérard Morel, Annie Cavalier.

Holman Biotech Commons QH452.8 .M6713 2001
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Format:
Book
Author/Creator:
Morel, Gérard.
Contributor:
Cavalier, Annie.
Series:
Methods in visualization
Standardized Title:
Hybridation in situ en microscopie photonique. English
Language:
English
French
Subjects (All):
In situ hybridization.
Microscopy.
In Situ Hybridization.
Medical Subjects:
In Situ Hybridization.
Microscopy.
Physical Description:
xiv, 327 pages : illustrations (some color) ; 26 cm.
Other Title:
Hybridization in light microscopy
Place of Publication:
Boca Raton, FL : CRC Press, 2001.
Summary:
In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used by molecular biologists, pathologists, geneticists, and biochemists. It will also appeal to research scientists who are interested in visualizing methods for nucleic acids and proteins. The book features a two-column layout for protocols/principles, simplifies the techniques and offers many schemes and tables to help the reader choose the best procedure.
Contents:
Chapter 1 Probes 1
Chapter 2 Tissue Preparation 61
Chapter 3 Pretreatments 89
Chapter 4 Hybridization 107
Chapter 5 Revelation of Hybrids 139
Chapter 6 Counterstaining and Modes of Observation 187
Chapter 7 Controls and Problems 207
Chapter 8 Typical Protocols 221.
Notes:
Includes bibliographical references and index.
ISBN:
0849307031
OCLC:
53227236

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