Basic methods in antibody production and characterization / edited by Gary C. Howard, Delia R. Bethell.

Format:

Book

Contributor:

Howard, Gary C.

Bethell, Delia R.

Language:

English

Subjects (All):

Immunoglobulins--Laboratory manuals.

Immunoglobulins.

Genre:

Laboratory manuals.

Physical Description:

271 pages : illustrations ; 24 cm

Place of Publication:

Boca Raton : CRC Press, 2001.

Summary:

First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications. Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

Contents:

Chapter 1 Starting Out / Delia R. Bethell, Gary C. Howard 1

Chapter 2 Animal Welfare and Regulatory Issues / Sigmund T. Rich 5

Chapter 3 Antigens / Donald H. Lewis 11

Chapter 4 Adjuvants / Jory R. Baldridge, Michael J. Lacy 19

Chapter 5 Polyclonal Antibodies / Eric S. Bean 31

Chapter 6 Monoclonal Antibody Production / Sandy J. Stewart 51

Chapter 7 Screening Techniques / Gamal E. Osman, Warren E. Ladiges 69

Chapter 8 Antibody Production by Ascites Growth / Kristi R. Harkins 77

Chapter 9 Growth of Cell Lines in Bioreactors / Beverly J. Norris, Michael J. Gramer, Mark D. Hirschel 87

Chapter 10 Phage Display / Peter Amersdorfer, James D. Marks 105

Chapter 11 Antibody Purification Methods / Kristi R. Harkins 141

Chapter 12 Molecular Characterization of Immunoglobulin Genes / Warren Ladiges, Gamal E. Osman 169

Chapter 13 Antibody Storage / Kathryn Elwell, Delia R. Bethell 193

Chapter 14 Chemically Modifying Antibodies / Kathryn Elwell, Gary C. Howard 199

Chapter 15 Western Blotting / Lee Bendickson, Marit Nilsen-Hamilton 217

Chapter 16 Immunohistochemical Methods / Kendrick J. Morrell, Neil M. Hand, Nicholas R. Griffin 239.

Notes:

Includes bibliographical references and index.

ISBN:

0849394457

OCLC:

44039615