Phage display : a laboratory manual / by Carlos F. Barbas III ... [and others].

Format:

Book

Contributor:

Barbas, Carlos F.

Alumni and Friends Memorial Book Fund.

Language:

English

Subjects (All):

Bacteriophages--Laboratory manuals.

Bacteriophages.

Microbial biotechnology--Laboratory manuals.

Microbial biotechnology.

Viral proteins--Laboratory manuals.

Viral proteins.

Affinity chromatography--Laboratory manuals.

Affinity chromatography.

Peptides--Biotechnology--Laboratory manuals.

Peptides.

Peptides--Biotechnology.

Proteins--Biotechnology--Laboratory manuals.

Proteins.

Proteins--Biotechnology.

Viral Proteins.

Chromatography, Affinity.

Biotechnology.

Medical Subjects:

Bacteriophages.

Microbial biotechnology.

Viral Proteins.

Chromatography, Affinity.

Peptides.

Biotechnology.

Genre:

Laboratory manuals.

Physical Description:

1 volume in various pagings : illustrations ; 27 cm

Place of Publication:

Cold Spring Harbor, NY : Cold Spring Harbor Labortory Press, 2000.

Summary:

Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria.

Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise and experience who attended a course on the technology at Cold Spring Harbor Laboratory.

Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

Contents:

Section 1 Phage Display

1 Filamentous Phage Biology / R. Webster 1

2 Phage-display Vectors / J.K. Scott, C.F. Barbas III 1

3 Antibody Libraries / D.R. Burton 1

4 Peptide Libraries / J.K. Scott 1

5 Functional Domains and Scaffolds / G.J. Silverman 1

6 Gene Fragment Libraries and Genomic and cDNA Expression Cloning / R.A. Williamson, G.J. Silverman 1

Section 2 Antibody Libraries

7 Overview: Amplification of Antibody Genes / D.R. Burton 1

8 Generation of Antibody Libraries: Immunization, RNA Preparation, and cDNA Synthesis / J. Andris-Widhopf, C. Rader, C.F. Barbas III 1

9 Generation of Antibody Libraries: PCR Amplification and Assembly of Light- and Heavy-chain Coding Sequences / J. Andris-Widhopf, P. Steinberger, R. Fuller, C. Rader, C.F. Barbas III 1

10 Selection from Antibody Libraries / C. Rader, P. Steinberger, C.F. Barbas III 1

11 Analysis of Selected Antibodies / P. Steinberger, C. Rader, C.F. Barbas III 1

12 Production and Purification of Fab and scFv / M. Elia, J. Andris-Widhopf, R. Fuller, C.F. Barbas III 1

13 Antibody Engineering / C. Rader, C.F. Barbas III 1

Section 3 Peptide Libraries

14 Overview: Peptide Libraries / J.K. Scott 1

15 General Phage Methods / L.L.C. Bonnycastle, A. Menendez, J.K. Scott 1

16 Production of Peptide Libraries / L.L.C. Bonnycastle, J. Shen, A. Menendez, J.K. Scott 1

17 Screening Peptide Libraries / A. Menendez, L.L.C. Bonnycastle, O.C.C. Pan, J.K. Scott 1

18 Analysis of Phage-borne Peptides / M.B. Zwick, A. Menendez, L.L.C. Bonnycastle, J.K. Scott 1

19 Construction and Use of pIII-displayed Peptide Libraries / K.A. Noren, L.H. Saltman, C.J. Noren 1

Section 4 Other Libraries and Other Methods of Panning

20 Construction and Selection from Gene Fragment Phage-display Expression Libraries / G.J. Silverman 1

21 Construction and Selection from cDNA Phage-display Expression Libraries / G.J. Silverman 1

22 In Vivo Selection of Phage-display Libraries / R. Pasqualini, W. Arap, D. Rajotte, E. Ruoslahti 1

23 Cell-surface Selection and Analysis of Monoclonal Antibodies from Phage Libraries / D.L. Siegel 1.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

ISBN:

0879695463

0879695455

OCLC:

59546462