Interpreting protein mass spectra : a comprehensive resource / A. Peter Snyder.

Format:

Book

Author/Creator:

Snyder, A. Peter, 1952-

Contributor:

Alumni and Friends Memorial Book Fund.

Language:

English

Subjects (All):

Proteins--Spectra.

Proteins.

Mass spectrometry.

Physical Description:

xi, 522 pages : illustrations ; 25 cm

Place of Publication:

Washington, D.C. : American Chemical Society ; Oxford ; New York : Oxford University Press, 2000.

Summary:

Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.

Contents:

1 Electrospray Ionization as a Vehicle for Protein Mass Spectra 3

2 Analyte Separation Methods: High-Pressure Liquid Chromatography and Capillary Electrophoresis 14

3 The Isotope Effect at High Mass 35

4 Calculation of the Analyte Mass 55

5 Analytical Figures of Merit for a High-Mass Isotope Envelope 87

6 Isotope Distribution of a Multiply Charged Mass Spectral Peak 111

7 Experimental Parameters Affecting Electrospray Ionization Mass Spectra 122

8 The Cation Effect on Electrospray Ionization Mass Spectra 144

9 Interpretation of Peptide and Protein Mass Spectra 155

10 Interpretation of Phosphoprotein Mass Spectra 265

11 Immunological Applications: Proteins and Phosphoproteins 300

12 Interpretation of Glycoprotein Mass Spectra 334.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

ISBN:

0841235716

OCLC:

41211435