The ELISA guidebook / by John R. Crowther.

Format:

Book

Contributor:

Crowther, J. R.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 149.

Methods in molecular biology ; v. 149

Language:

English

Subjects (All):

Enzyme-linked immunosorbent assay.

Enzyme-Linked Immunosorbant Assay.

Medical Subjects:

Enzyme-Linked Immunosorbant Assay.

Physical Description:

xi, 421 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, NJ : Humana Press, 2001.

Summary:

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.

Contents:

1 Overview of ELISA in Relation to Other Disciplines 1

2 Systems in ELISA 9

3 Stages in ELISA 45

4 Titration of Reagents 83

5 Theoretical Considerations 115

6 Practical Exercises 153

7 Monoclonal Antibodies 233

8 Validation of Diagnostic Tests for Infectious Diseases 301

9 Charting Methods for Internal Quality Control 347

10 Immunochemical Techniques 395

11 Test Questions 407.

Notes:

Includes bibliographical references and index.

ISBN:

0896037282

OCLC:

43060501