Chemokine protocols / edited by Amanda E.I. Proudfoot, Timothy N.C. Wells, and Christine A. Power.

Format:

Book

Contributor:

Proudfoot, Amanda E. I.

Wells, Timothy N. C.

Power, Christine A.

Series:

Methods in molecular biology ; v. 138.

Methods in molecular biology ; v. 138

Language:

English

Subjects (All):

Chemokine--Research--Methodology.

Chemokine.

Chemokines--analysis.

Chemokines--physiology.

Receptors, Chemokine--analysis.

Medical Subjects:

Chemokines--analysis.

Chemokines--physiology.

Receptors, Chemokine--analysis.

Physical Description:

xiii, 353 p : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, 2000.

Contents:

1 Purification of Chemokines from Natural Sources / Jens-M. Schroder 1

2 Cloning of Novel Chemokines Using a Signal Sequence Trap Method / Toshio Imai 11

3 Chemokine Expression in Insect Cells / Toshio Imai 23

4 Expression of Chemokines in Escherichia coli / Michael D. Edgerton, Lars-Ole Gerlach, Thomas P. Boesen, Bernard Allet 33

5 Expression of Chemokines in the Periplasmic Space of E. coli / Jochen Pfirstinger, Matthias Mack 41

6 Synthesis of Chemokines / Ian Clark-Lewis 47

7 Identification of Novel Chemokines from Expressed Sequence Tag Databases / Timothy N. C. Wells, Manuel C. Peitsch 65

8 Purification of Recombinant Chemokines from E. coli / Amanda E. I. Proudfoot, Frederic Borlat 75

9 Chemokine Receptor Cloning / Philip M. Murphy 89

10 Generation of Stable Cell Lines Expressing Chemokine Receptors / Christine A. Power, Alexandra Meyer 99

11 Modified Microchemotaxis Assays / Dennis D. Taub 105

12 Transwell Chemotaxis / Paul D. Ponath, Juan Wang, Heidi Heath 113

13 Endothelial Cell Chemotaxis Assays / Darcey Black 121

14 Radiolabeled Chemokine Binding Assays / Bruce L. Daugherty, Salvatore J. Siciliano, Martin S. Springer 129

15 Scintillation Proximity Binding Assay / Sami Alouani 135

16 Calcium Mobilization / Raphaele Buser, Amanda E. I. Proudfoot 143

17 Actin Polymerization / Jorn Elsner, Alexander Kapp 149

18 Reactive Oxygen Release / Jorn Elsner, Alexander Kapp 153

19 Histamine Release / Michael A. Lett-Brown, Rafeul Alam 157

20 Measurement of Phosphoinositide 3-Kinase Activity / Stephen G. Ward 163

21 Glycosaminoglycan Binding Assays / Arlene J. Hoogewerf, Gabriele S. V. Kuschert 173

22 CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoietic Progenitors / Gerard J. Graham, Mary G. Freshney 179

23 Downmodulation and Recycling of Chemokine Receptors / Matthias Mack, Detlef Schlondorff 191

24 Analysis of Chemokine Receptor Endocytosis and Recycling / Nathalie Signoret, Mark Marsh 197

25 Chemokine Inhibition of HIV Infection / Jacqueline D. Reeves, Graham Simmons 209

26 The Production of Chemokine Specific Monoclonal Antibodies: Chemokine RANTES / Peter J. Nelson 223

27 Monoclonal Antibodies to Chemokine Receptors / Paul D. Ponath, Nasim Kassam, Shixin Qin 231

28 Targeted Expression of Chemokines In Vivo / Iqbal Grewal, Long Gu, Susan Tseng, Barrett J. Rollins 243

29 Chemokine Knockout Mice / Marc E. Rothenberg 253

30 Chemokine Receptor Knockout Mice / Ji-Liang Gao, Philip M. Murphy 259

31 Measurement of Eosinophil Accumulation In Vivo / Maria-Jesus Sanz, Peter J. Jose, Timothy J. Williams 275

32 Murine Model of Allergic Lung Inflammation / Sami Alouani, Pierre Juillard, Yolande Chvatchko 285

33 Murine Models of Airway Inflammation / Emma M. Campbell, Nicholas W. Lukacs 295

34 Rat Models of Respiratory Inflammation / Andrew S. McWilliam, Patrick G. Holt 303

35 Murine Model of Crescentic Nephritis / Clare Lloyd, Jose-Carlos Gutierrez-Ramos 311

36 Rabbit Models of Pneumonia, Peritoneal Sepsis, and Lung Injury / Charles W. Frevert, Gustavo Matute-Bello, Thomas R. Martin 319.

Notes:

Includes bibliographical references and index.

ISBN:

0896037223

OCLC:

42862013