Imaging neurons : a laboratory manual / edited by Rafael Yuste, Frederick Lanni, Arthur Konnerth.

Format:

Book

Contributor:

Yuste, Rafael.

Lanni, Frederick.

Konnerth, Arthur.

Language:

English

Subjects (All):

Neurons.

Microscopy.

Medical Subjects:

Neurons.

Microscopy.

Physical Description:

1 volume (various pagings) : illustrations (some color) ; 26 cm

Place of Publication:

Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, [2000]

Contents:

Section 1 Microscopes, Image Acquisition, and the Basics of Fluorescence Imaging

1. Microscopy and Microscope Optical Systems / F. Lanni, H.E. Keller 1

2. Arc Lamps and Monochromators for Fluorescence Microscopy / R. Uhl 1

3. The Use of Liquid-crystal Tunable Filters for Fluorescence Imaging / K.R. Spring 1

4. Acousto-optic Tunable Filters for Microscopy / E.S. Wachman 1

5. Video Microscopy, Video Cameras, and Image Enhancement / Reprint (modified) from Cells: A Laboratory Manual 1

6. The Application of Scientific-grade CCD Cameras to Biological Imaging / M. Christenson 1

7. Infrared Videomicroscopy / H.-U. Dodt, G. D'Arcangelo, W. Zieglgansberger 1

8. Grating Image Systems for Optical Sectioning Fluorescence Microscopy of Cells, Tissues, and Small Organisms / F. Lanni, T. Wilson 1

9. Analysis of Dynamic Optical Imaging Data / P.P. Mitra, B. Pesaran, D. Kleinfeld 1

10. Maintaining Live Cells and Tissue Slices in the Imaging Setup / M.E. Dailey 1

Section 2 Confocal Microscopy

11. Confocal Microscopy: Principles and Practice / A. Fine 1

12. Practical Limits to Resolution in Fluorescence Light Microscopy / E.H.K. Stelzer 1

13. In Vivo Imaging of CNS Neuron Development / H.T. Cline, J.E. Edwards, I. Rajan, G.-Y. Wu, D.-J. Zou 1

14. The Zebrafish Hindbrain: A Transparent System for Imaging Motor Circuitry / D.M. O'Malley, J.R. Fetcho 1

15. Fast Confocal Fluorescence Imaging of Subcellular Calcium Dynamics / J. Eilers 1

16. Imaging Calcium Sparks in Excitable Cells / M.B. Cannell, C. Soeller 1

Section 3 Multiphoton Microscopy

17. Principles of Multiphoton-excitation Fluorescence Microscopy / W. Denk 1

18. Lasers for Multiphoton Microscopy / F.W. Wise 1

19. Two-photon Cross Sections of Indicators / C. Xu 1

20. Two-photon Microscopy for 40 Imaging of Living Neurons / S.M. Potter 1

21. Multiphoton Imaging of Embryonic Development / W.A. Mohler, J.M. Squirrell 1

22. Two-photon Imaging of Neuronal Function in the Neocortex In Vivo / K. Svoboda, D.W. Tank, R.A. Stepnoski, W. Denk 1

23. Two-photon Imaging of Neocortical Microcirculation / D. Kleinfeld, W. Denk 1

Section 4 Photoactivation

24. Basics of Photoactivation / G.C.R. Ellis-Davies 1

25. Design and Application of Caged Neurotransmitters / G.P. Hess 1

26. Uncaging Calcium in Neurons / K.R. Delaney 1

27. Photostimulation with Caged Glutamate / K. Kandler, R.S. Givens, L.C. Katz 1

28. Infrared-guided Photostimulation of Neurons in Brain Slices / H.-U. Dodt, A. Frick, W. Zieglgansberger 1

29. Spatially Resolved Flash Photolysis Via Chemical Two-photon Uncaging / G.J. Augustine, D.L. Pettit, S.S.-H. Wang 1

Section 5 Calcium Imaging

30. How Calcium Indicators Work / S.R. Adams 1

31. Some Quantitative Aspects of Calcium Fluorimetry / E. Neher 1

32. Calibration of Fluorescent Calcium Indicators / F. Helmchen 1

33. A Single-compartment Model of Calcium Dynamics in Nerve Terminals and Dendrites / F. Helmchen, D.W. Tank 1

34. Loading Brain Slices with AM Esters of Calcium Indicators / R. Yuste 1

35. Dye Loading with Patch Pipets / J. Eilers, A. Konnerth 1

36. Measuring Calcium Fluxes in Dendritic Domains of Neurons in Brain Slices / O. Garaschuk, A. Konnerth 1

37. Monitoring Presynaptic Calcium Dynamics with Membrane-permeant Indicators / W.G. Regehr 1

38. Imaging Intracellular Calcium-concentration Microdomains at a Chemical Synapse / R. Linas, M. Sugimori 1

39. Monitoring Intramitochondrial Calcium with Rhod-2 / M. Hoth, R.S. Lewis 1

40. Generation of Controlled Calcium Oscillations in Nonexcitable Cells / R.E. Dolmetsch, R.S. Lewis 1

41. Calcium Imaging of Retinal Activity / R.O.L. Wong 1

42. Calcium Imaging of Identified Astrocytes in Hippocampal Slices / J. Kang, M. Nedergaard 1

43. High-speed Calcium Imaging of Neurons in Brain Slices / W.N. Ross, R. Gray, N. Lasser-Ross 1

44. In Vivo Calcium Imaging in Blowfly CNS / A. Borst, S. Single 1

Section 6 Imaging Other Aspects of Neuronal Function

45. Intrinsic Signal Imaging in the Neocortex / A. Grinvald, A. Shmuel, I. Vanzetta, E. Shtoyerman, D. Shoham, A. Arieli 1

46. Intrinsic Signal Optical Imaging as a Tool to Visualize the Development of Functional Maps in the Mammalian Visual Cortex / T. Bonhoeffer 1

47. Intrinsic Signal Optical Imaging in Brain Slices / B.A. MacVicar 1

48. Rapid Changes in Light Scattering Associated with Secretion from Petidergic Nerve Terminals / B.M. Salzberg, M. Muschol, A.L. Obaid 1

49. Imaging with Voltage-sensitive Dyes: Spike Signals, Population Signals, and Retrograde Transport / J.-Y. Wu, L.B. Cohen, Y. Tsau, Y.-W. Lam, M. Zochowski, C.X. Falk 1

50. Voltage-sensitive-dye Imaging in the Neocortex: Visualization of Coherent Neuronal Assemblies / A. Grinvald, R. Hildesheim, D. Shoham, D.E. Glaser, A. Arieli 1

51. Imaging Membrane-potential Changes with Intracellular Voltage-sensitive Dyes / S. Antic, D. Zecevic 1

52. Imaging Sodium Dynamics in Neurons / W.N. Ross, J.C. Callaway, N. Lasser-Ross 1

53. Imaging Synaptic Vesicle Dynamics at the Frog Neuromuscular Junction with the Styryl Dye FM1-43 / U. Becherer, J.K. Angleson, W.J. Betz 1

54. Imaging Exocytosis with Total Internal Reflection Microscopy / J.A. Steyer, W. Almers 1

Section 7 Genetically Engineered Fluorescent Probes

55. Physiological Indicators Based on Fluorescence Resonance Energy Transfer / R.Y. Tsien 1

56. Cellular Imaging of Bioluminescence / J.D. Plautz, S.A. Kay 1

57. Green Fluorescent Proteins for Measuring Signal Transduction: A Voltage-sensor Prototype / M.S. Siegel, E.Y. Isacoff 1

58. Introduction of Green Fluorescent Protein into Hippocampal Neurons through Viral Infection / R. Malinow, Y. Hayashi, M. Maletic-Savatic, S.H. Zaman, J.-C. Poncer, S.-H. Shi, J.A. Esteban 1

59. Imaging Exocytosis with pH-sensitive Green Fluorescent Proteins / G. Miesenbock 1

60. Targeted Recombinant Aequorins: New Tools in the Study of Calcium Homeostasis / T. Pozzan, R. Rizzuto 1

Appendix 2 Electromagnetic Spectrum 2

Appendix 3 Microscopy: Lenses, Filters, and Emission/Excitation Spectra 3

Appendix 6 How to Calculate and Prepare Calcium-EGTA Buffers 6.

Notes:

Includes bibliographical references and index.

ISBN:

0879695420

0879695412

OCLC:

42289885