High resolution chromatography : a practical approach / edited by Paul Millner.

Format:

Book

Contributor:

Millner, Paul.

Anne and Joseph Trachtman Memorial Book Fund.

Series:

Practical approach series ; 204.

Practical approach series ; 204

Language:

English

Subjects (All):

Affinity chromatography.

Biomolecules--Purification.

Biomolecules.

Proteins--Purification.

Proteins.

Physical Description:

xx, 311 pages : illustrations ; 25 cm.

Place of Publication:

Oxford ; New York : Oxford University Press, 1999.

Summary:

Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Anne and Joseph Trachtman Memorial Book Fund.

ISBN:

0199636494

0199636486

OCLC:

40200143