2-D proteome analysis protocols / edited by Andrew J. Link.

Format:

Book

Contributor:

Link, Andrew J.

Alumni and Friends Memorial Book Fund.

Series:

Methods in molecular biology (Clifton, N.J.) ; 112.

Methods in molecular biology ; vol. 112

Language:

English

Subjects (All):

Proteins--Analysis.

Proteins.

Gel electrophoresis.

Gene expression--Research--Methodology.

Gene expression.

Proteins--analysis.

Gene Expression.

Medical Subjects:

Proteins--analysis.

Gene Expression.

Physical Description:

xvii, 601 pages : illustrations ; 24 cm.

Other Title:

Two-D proteome analysis protocols

Place of Publication:

Totowa, N.J. : Humana Press, [1999]

Summary:

This practical lab manual is designed for any molecular biology or clinical lab wishing to use 2-D to analyze the global protein content of cells or tissue. The book's contributors include many leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectrometry-based protein identification methodologies.

The researcher is guided through the complete process of using 2-D protein gels from making the protein extract to finally identifying the proteins. It includes protocols for working with all of the model organisms including bacteria, yeast, nematode, fruit fly, plants, mouse and human. In line with the growing importance of the Internet, protocols for comparing results with other Internet-based 2-D databases are provided, including a method for constructing your own 2-D database Web site. As the most comprehensive and authoritative source on 2-D Proteome analysis, this book will become the standard reference in the field for use by experts and beginners alike.-- Includes chapters by those who pioneered the field including Klose and Gorg, as well as from contemporary leaders including Rabilloud, Hochstrasser, Appel, Yates, and Mann-- The technique is very accurate for the identification of virtually all proteins in a cell or tissue, and step-by-step methods bring this technique to any lab using 2-D Gels

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

ISBN:

0896035247

OCLC:

39633795