Essential molecular biology : a practical approach / edited by T.A. Brown.

Format:

Book

Contributor:

Brown, T. A. (Terence A.)

Series:

Practical approach series

The Practical approach series

Language:

English

Subjects (All):

Molecular cloning.

Recombinant DNA.

Cloning, Molecular.

DNA, Recombinant.

Medical Subjects:

Cloning, Molecular.

DNA, Recombinant.

Physical Description:

2 volumes : illustrations ; 24 cm.

Place of Publication:

Oxford ; New York : IRL Press at Oxford University Press, [1991]

Summary:

The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Volume II covers the procedures needed for identification of specific genes and the study of their structure and cellular expression. Topics covered include preparation of gene libraries, hybridization methods, nucleic acid sequencing, DNA mapping, and DNA amplification by the polymerase chain reaction. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book thus brings powerful techniques at the forefront of molecular biology research to the researcher. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.

Notes:

Includes bibliographical references and indexes.

ISBN:

019963114X

0199631158

0199631115

0199631107

0199631123

0199631131

OCLC:

22542731