Tumor marker protocols / edited by Margaret Hanausek and Zbigniew Walaszek.

Format:

Book

Contributor:

Hanausek, Margaret.

Walaszek, Zbigniew.

Series:

Methods in molecular medicine ; 14.

Methods in molecular medicine ; 14

Language:

English

Subjects (All):

Tumor markers.

Biomarkers, Tumor.

Neoplasms--diagnosis.

Medical Subjects:

Biomarkers, Tumor.

Neoplasms--diagnosis.

Physical Description:

xiv, 500 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [1998]

Summary:

A much-needed collection of cutting-edge methods for the detection and discovery of new cancer markers, particularly molecular tumor markers. Each method is fully described by a hands-on expert who regularly uses it in the laboratory and has perfected it to a high degree of ready reproducibility. Special emphasis is given to statistical considerations in the analysis of tumor markers, recent discoveries in the field of serum and tissue marker proteins, cytogenetic markers, molecular markers, and markers for the early detection of cancer. The well-honed procedures detailed here will enable cancer researchers and clinical oncologists alike to realize the promise of these powerful new diagnostic strategies for the detection, therapy, and successful prevention of cancer.

Contents:

Part I. Multiple Markers: Principles and Guidelines

1 Integrating Multiple Clinical Tests to Increase Predictive Power / Harry B. Burke 3

2 Statistical Considerations in the Analysis of Tumor Markers / Dennis A. Johnston 11

3 Selection and Development of Biomarkers for Bladder Cancer / George P. Hemstreet, III, Robert E. Hurst, Rebecca B. Bonner 37

4 Clinical Application of Tissue and Serum Markers in Breast Cancer / Gordon F. Schwartz, Roland Schwarting 61

Part II. Immunodiagnosis of Cancer: Serum and Tissue Markers

5 Serum and Tissue Biomarkers in the Prognosis and Treatment of Breast Cancer / Tina J. Hieken, Rajeshwari R. Mehta 95

6 The Oncofetal Protein p65 in Breast Cancer Detection / Margaret Hanausek, Zbigniew Walaszek, Ute Sherman, Jerzy T. Klijanienko 103

7 Determination of Tumor Ferritin Concentration in Breast Cancer / Jonathan F. Head, Robert L. Elliott 115

8 Immunodiagnosis of Childhood Malignancies / David M. Parham, Hallie Holt 127

9 Immunohistochemical Evaluation of Biomarkers in Prostatic and Colorectal Neoplasia: Principles and Guidelines / William E. Grizzle, Russell B. Myers, Upender Manne, Sudhir Srivastava 143

10 Factors Affecting Immunohistochemical Evaluation of Biomarker Expression in Neoplasia / William E. Grizzle, Russell B. Myers, Upender Manne, Cecil Rob Stockard, Lualhati E. Harkins, Sudhir Srivastava 161

11 Instrumentation, Accuracy, and Quality Control Issues in Development of Quantitative Fluorescence-Image Analysis (QFIA) / Rebecca B. Bonner, Robert E. Hurst, Jian Yu Rao, George P. Hemstreet 181

Part III. Cytogenetic Markers

12 Cytogenetics as a Diagnostic Aid for Childhood Hematologic Disorders: Conventional Cytogenetic Techniques, Fluorescence In Situ Hybridization, and Comparative Genomic Hybridization / Susanna C. Raimondi, Susan Mathew, Ching-Hon Pui 209

13 Preparation of Metaphase Chromosomes for Cytogenetic Analysis / Kevin A. Hahn, Penny K. Riggs 229

14 Chromosome Staining and Banding Techniques / Kevin A. Hahn, Penny K. Riggs 239

15 Fluorescence In Situ Hybridization to Chromosomes / Penny K. Riggs, Kevin A. Hahn 245

16 Detection of Rearrangements in the bc1-2 Gene Using the Polymerase Chain Reaction / Ruth W. Craig, Dorothy R. Belloni, Ernest S. Kawasaki, Norman B. Levy 255

Part IV. Molecular Markers

17 Applications of Tissue Microdissection in Molecular Pathology: Principles and Guidelines / Michael R. Emmert-Buck, Irina A. Lubensky, Rodrigo F. Chuaqui, Larisa V. Debelenko, Cathy D. Vocke, Maria J. Merino, Paul H. Duray, W. Marston Linehan, Lance A. Liotta, Zhengping Zhuang 269

18 EWS Gene Fusions as Diagnostic Markers in Sarcomas: Principles and Guidelines / Marc Ladanyi 299

19 p53 Detection in Breast Cancer / Penelope L. Davis, J. Dirk Iglehart 319

20 Use of the Polymerase Chain Reaction Technique to Detect the t(14;18) Translocation in Lymphoid Tissue / Maryalice Stetler-Stevenson, Megan S. Lim 331

21 Detection of ras Gene Mutations Using Oligonucleotide Ligation Technology / Faye A. Eggerding 341

22 Detection of Prostate-Cancer Cells in Blood and Bone Marrow by RT-PCR / Robert L. Vessella, Eva Corey 357

23 Quantitative, Competitive RT-PCR Analysis of Biomarkers in the Study of Neoplasia / Richard D. Hockett, Jr., Miriam D. Rogers, Sudhir Srivastava 373

24 Differential Display to Define Molecular Markers and Genes That Mediate Malignancy / Edward J. Pavlik, Katherine Nelson, Suseela Srinivasan, Thomas L. Johnson, Paul D. DePriest 391

Part V. Miscellaneous Markers and Early Detection of Cancer

25 Transforming Growth Factor Beta: A Plasma Tumor Marker / Feng-Ming Kong, Mitchell S. Anscher, Randy L. Jirtle 417

26 Anti-HMdU Autoantibodies in Human Sera as a Biomarker of Cancer Risk / Krystyna Frenkel, Jerzy Karkoszka 431

27 A Scientific Basis for Cancer Prevention: Defining the Role of Individual Cytosolic GST Isozyme / Sanjay K. Srivastava, Sanjay Awasthi 447

28 Aberrant Crypt Foci System to Study Cancer Preventative Agents in the Colon: Principles and Guidelines / Ranjana P. Bird 465

29 Strain-Dependent Differences in the Expression of the Oncofetal Protein p65 in Mice Susceptible and Resistant to Chemical Carcinogenesis / Janusz Szemraj, Margaret Hanausek, Zbigniew Walaszek, Alan K. Adams 475

30 D-Glucaric Acid as a Prospective Tumor Marker / Zbigniew Walaszek, Margaret Hanausek, Janusz Szemraj, Alan K. Adams 487.

Notes:

Includes bibliographical references and index.

Local Notes:

Erratum tipped-in at p. xiv.

ISBN:

0896033805

OCLC:

38140224