Clinical applications of PCR / edited by Y.M. Dennis Lo.

Format:

Book

Contributor:

Lo, Y. M. Dennis.

Series:

Methods in molecular medicine ; 16.

Methods in molecular medicine ; 16

Language:

English

Subjects (All):

Polymerase chain reaction--Diagnostic use.

Polymerase chain reaction.

Polymerase Chain Reaction--methods.

Medical Subjects:

Polymerase Chain Reaction--methods.

Physical Description:

x, 353 pages : illustrations ; 24 cm.

Place of Publication:

Totowa, N.J. : Humana Press, [1998]

Summary:

An unprecedented collection of core PCR techniques useful for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. The book demonstrates over and over again the power of PCR -- its high sensitivity, specificity, and ability to rapidly discriminate sequence variations.-- Requires little previous experience to successfully reproduce the methods

Contents:

1 Introduction to the Polymerase Chain Reaction / Y. M. Dennis Lo 3

2 Setting Up a PCR Laboratory / Y. M. Dennis Lo 11

3 Amplification from Archival Materials / Y. M. Dennis Lo 21

4 Quantitative PCR / Luc Raeymaekers 27

5 Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols / Philip J. Saker 39

6 Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal Labeling / Hiroyuki Iwahana, Mitsuo Itakura 51

7 The Amplification Refractory Mutation System / Y. M. Dennis Lo 61

8 Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis / Y. M. Dennis Lo, Virginia A. Horton 71

9 Long Range PCR / William Waggott 81

10 Generation of Labeled Probes by PCR / Y. M. Dennis Lo, Shu F. An 93

11 PCR for the Detection of Minority DNA Populations / Y. M. Dennis Lo 101

12 In Situ Amplification / John O' Leary 109

13 Sequencing of PCR Products / Paul Moss, Swee Lay Thein 145

14 Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) / Rajko Kusec 149

15 PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation / Etienne Roux 161

16 Diagnosis of Mitochondrial Disorders Using the PCR / Karl J. Morten 171

17 PCR Analysis of CD44 Variants in Tumors / John Bolodeoku 189

18 Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction / Susan A. Burchill 203

19 Detection of Circulating Tumor Cells Using Immunobead-PCR / Jennifer E. Hardingham 225

20 Single Cell PCR: Theory, Practice, and Clinical Applications / Ian Findlay 233

21 PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation / Y. M. Dennis Lo 265

22 Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood / Akihiko Sekizawa, Hiroshi Saito, Takumi Yanaihara 275

23 Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescent PCR / Barbara Pertl, Matteo Adinolfi 287

24 Detection of the Hepatitis C Virus by RT-PCR / Christopher John Healy, Steven Read 301

25 The Application of PCR to the Detection of M. tuberculosis in Sputum Samples / Majella Maher, Maura Glennon, Martin Cormican, Terry Smith 309

26 Microsatellite Analysis in Human Disease / John Koreth, Christopher J. Bakkenist, James O' D. McGee 321.

Notes:

Includes bibliographical references and index.

ISBN:

0896034992

0896033597

OCLC:

38140223