Recombinant DNA principles and methodologies / edited by James J. Greene, Venigalla B. Rao.

Format:

Book

Contributor:

Greene, James J. (James Joseph)

Rao, Venigalla B.

Alumni and Friends Memorial Book Fund.

Language:

English

Subjects (All):

Molecular cloning.

Recombinant DNA.

Cloning, Molecular.

DNA, Recombinant.

Medical Subjects:

Cloning, Molecular.

DNA, Recombinant.

Physical Description:

ix, 750 pages : illustrations ; 24 cm

Place of Publication:

New York : Marcel Dekker, [1998]

Summary:

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Notes:

Includes bibliographical references and index.

Local Notes:

Acquired for the Penn Libraries with assistance from the Alumni and Friends Memorial Book Fund.

ISBN:

0824799895

OCLC:

38965991