Bioseparation of proteins : unfolding/folding and validations / Ajit Sadana.

Format:

Book

Author/Creator:

Sadana, Ajit, 1947-

Series:

Separation science and technology (San Diego, Calif.) ; v. 1.

Separation science and technology ; v. 1

Language:

English

Subjects (All):

Proteins--Biotechnology.

Proteins--Separation.

Protein folding.

Physical Description:

xx, 346 pages : illustrations ; 27 cm.

Place of Publication:

San Diego : Academic Press, [1998]

Summary:

This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.

Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biological product) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.

Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption, process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.

Contents:

Steps in bioseparation processes

High-resolution fractionation processes

Interfacial protein adsorption and inactivation during bioseparation

Protein inactivations during chromatographic methods of separation

Protein inactivations during novel bioseparation techniques

Adsorption influence on bioseparation and inactivation

Applications and economics of bioseparation

Protein refolding and inactivation during bioseparation

Validation of the production of biological products.

Notes:

Includes bibliographical references and index.

ISBN:

0126140405

OCLC:

38155636