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Molecular correlates of chronic corticosterone exposure in the hippocampus / Suresh Mohanchandran Nair.
LIBRA Thesis N158 1996
Available from offsite location
LIBRA Diss. POPM1996.378
Available from offsite location
- Format:
- Book
- Manuscript
- Microformat
- Thesis/Dissertation
- Author/Creator:
- Nair, Suresh Mohanchandran.
- Language:
- English
- Subjects (All):
- Penn dissertations--Pharmacology.
- Pharmacology--Penn dissertations.
- Academic Dissertations as Topic.
- Medical Subjects:
- Academic Dissertations as Topic.
- Local Subjects:
- Penn dissertations--Pharmacology.
- Pharmacology--Penn dissertations.
- Physical Description:
- xi, 167 pages : illustrations ; 29 cm
- Production:
- 1996.
- Summary:
- Corticosterone (CORT) binds to mineralocorticoid (MRs) and glucocorticoid receptors (GRs) in CA1 and CA3 hippocampal neurons, which respond to a wide range of CORT concentrations. Ligand-bound corticosteroid receptors regulate gene expression and alter levels of multiple mRNAs and proteins, thereby influencing basic neuronal properties e.g. membrane potential and Ca-influx. Exposure to high doses of CORT appears to be harmful to neuronal integrity.
- Initially, we employed in-situ transcription followed by antisense RNA amplification to characterize CORT-mediated regulation of gene expression in hippocampal sub-regions. Over 4 weeks, no alterations in expression levels of MR or GR mRNAs were seen in the CA1 or CA3. Expression of GLUT-3 mRNA was suppressed in both areas, suggesting a molecular mechanism for inhibition of neuronal glucose uptake by CORT. At 3 weeks, the CA3 expressed a two-fold ratio of NR2A:NR2B mRNA, and may consequently exhibit restricted NMDA receptor-mediated Ca-influx relative to the CA1.
- Next, voltage-gated Ca currents were recorded and mRNA expression characterized in individual CA1 neurons to identify regulation of Ca channel mRNAs by CORT.
- Notes:
- Adviser: James H. Eberwine.
- Thesis (Ph.D. in Pharmacology) -- University of Pennsylvania, 1996.
- Includes bibliographical references.
- Local Notes:
- University Microfilms order no.: 97-12981.
- OCLC:
- 187469411
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