Protein purification process engineering / edited by Roger G. Harrison.

Format:

Book

Contributor:

Harrison, Roger G., 1944-

Series:

Bioprocess technology ; v. 18.

Bioprocess technology ; v. 18

Language:

English

Subjects (All):

Proteins--Biotechnology.

Proteins.

Proteins--Purification.

Physical Description:

xiv, 381 pages : illustrations ; 24 cm.

Place of Publication:

New York : M. Dekker, 1994.

Summary:

This authoritative reference offers outstanding coverage of the development of protein purification processes for large-scale commercial operation and addresses process development, scale-up, applications, and mathematical descriptions. Covering many crucial technologies currently used at the commercial scale, Protein Purification Process Engineering discusses in detail analytical considerations in the development of protein purification processes ... examines the latest advances in crossflow membrane filtration, emphasizing the use of microfiltration for separating cells and cell debris ... supplies in-depth analyses of the precipitation of proteins, highlighting protein solubility and the unit operations employed ... provides practical information on the mechanical design and operation of chromatography columns ... furnishes an extensive study of up-to-the-minute developments in affinity chromatography ... presents a comprehensive treatment of freeze drying with an emphasis on process monitoring and equipment ... and more. Containing helpful figures and tables and over 1300 literature citations, Protein Purification Process Engineering is an invaluable resource for process, process development, bioprocess, biochemical, chemical, development, biochemical development, and plant engineers; applied, industrial, analytical, and bioanalytical chemists and biochemists; applied biologists; biotechnologists; industrial microbiologists; and all upper-level undergraduate, graduate, and continuing-education courses in biochemical engineering, bioseparations, and biochemical separations.

Contents:

1 Organization and Strategy / Roger G. Harrison 1

2 Analytical Considerations in the Development of Protein Purification Processes / Vincent Anicetti, William S. Hancock 11

3 Cell Breakage / Cady R. Engler 37

4 Crossflow Membrane Filtration / Zhi-Guo Su, Clark K. Colton 57

5 Liquid-Liquid Extraction / Dennis J. Kubek 87

6 Differential Precipitation of Proteins: Science and Technology / Fred Rothstein 115

7 Conventional Chromatography / John M. Simpson 209

8 Biospecific Affinity Chromatography / Nikos K. Harakas 259

9 Freeze Drying: A Practical Overview / Larry A. Gatlin, Steven L. Nail 317.

Notes:

Includes bibliographical references and index.

ISBN:

082479009X

OCLC:

28722772