Molecular methods for microbial identification and typing / K.J. Towner and A. Cockayne.

Format:

Book

Author/Creator:

Towner, K. J.

Contributor:

Cockayne, A. (Alan)

Language:

English

Subjects (All):

Bacteria--Classification--Technique.

Bacteria.

Microbiological Techniques.

Bacteriology--Classification.

Technique.

Medical Subjects:

Microbiological Techniques.

Physical Description:

202 pages : illustrations ; 24 cm

Edition:

First edition.

Place of Publication:

London ; New York : Chapman & Hall, [1993]

Summary:

The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have been concealed in scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages.

Contents:

Introduction to microbial identification and typing methods

Brief overview of microbial identification

Conventional biological methods for microbial typing

Molecular approaches to microbial identification and typing

Analysis of nucleic acid profiles

Plasmid analysis

Analysis of chromosomal DNA

Identification and typing by nucleic acid hybridization techniques

Basic principles of nucleic acid hybridization

Analysis of RFLPs by hybridization techniques

Nucleic acid amplification and sequencing techniques

Polymerase chain reaction (PCR)

Other amplification methods

Nucleic acid sequencing techniques

Analysis of protein and lipopolysaccharide profiles

Introduction to protein fingerprinting

Protein analysis

Lipopolysaccharide typing schemes

Typing and identification of microorganisms with antibodies

Production of antibodies for serotyping and identification

Techniques used in serotyping.

Notes:

Includes bibliographical references and index.

ISBN:

041249390X

OCLC:

29468427