Guide to electroporation and electrofusion / Donald C. Chang ... [and others].

Format:

Book

Contributor:

Chang, Donald C.

Language:

English

Subjects (All):

Electroporation.

Electrofusion.

Cytological Techniques.

Medical Subjects:

Cytological Techniques.

Physical Description:

x, 581 pages : illustrations ; 24 cm

Place of Publication:

San Diego : Academic Press, 1992.

Summary:

Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Key Features* Covers fundamentals of electroporation and electrofusion in detail* Molecular events* Mechanisms* Kinetics* Gives extensive practical information* The latest applications* Controlling parameters to maximize efficiency* Available instrumentation* Presents applications of electroporation and electrofusion in current research situations* State-of-the-art modifications to electrical pulses and generators* Application of electroporation and electrofusion to unique, alternative cell and tissue types* Gives straightforward, detailed, easy-to-follow protocols for* Formation of human hybridomas* Introduction of genetic material into plant cells and pollen* Transfection of mammalian cells* Transformation of bacteria, plants, and yeast* Production of altered embryos* Optimization of electroporation by using reporter genes* Comprehensive and up-to-date* Convenient bench-top format* Approximately 125 illustrationscomplement the text* Complete references with article titles* Written by leading authorities in electroporation and electrofusion

Contents:

Part I Mechanisms and Fundamental Processes in Electroporation and Electrofusion

2. Structure and Dynamics of Electric Field-Induced Membrane Pores as Revealed by Rapid-Freezing Electron Microscopy / Donald C. Chang 9

3. Events of Membrane Electroporation Visualized on a Time Scale from Microsecond to Seconds / Kazuhiko Kinosita, Jr., Masahiro Hibino, Hiroyasu Itoh, Masaya Shigemori, Ken'ichi Hirano, Yutaka Kirino, Tsuyoshi Hayakawa 29

4. Time Sequence of Molecular Events in Electroporation / Tian Y. Tsong 47

5. Electropores in Lipid Bilayers and Cell Membranes / Leonid V. Chernomordik 63

6. Biophysical Considerations of Membrane Electroporation / Eberhard Neumann, Andreas Sprafke, Elvira Boldt, Hendrik Wolf 77

7. Progress toward a Theoretical Model for Electroporation Mechanism: Membrane Electrical Behavior and Molecular Transport / James C. Weaver, Alan Barnett 91

8. Mechanisms of Electroporation and Electrofusion / Arthur E. Sowers 119

9. Interfacial Membrane Alteration Associated with Electropermeabilization and Electrofusion / J. Teissie, M.-P. Rols 139

10. Membrane Fusion Kinetics / Dimiter S. Dimitrov 155

11. Effects of Intercellular Forces on Electrofusion / Sek Wen Hui, David A. Stenger 167

12. Dynamics of Cytoskeletal Reorganization in CV-1 Cells during Electrofusion / Qiang Zheng, Donald C. Chang 179

Part II Applications of Electroporation and Electrofusion in Current Research

13. Gene Transfer into Adherent Cells Growing on Microbeads / Huntington Potter, Stefan W. F. Cooke 201

14. Gene Targeting and Electroporation / Laura H. Reid, Oliver Smithies 209

15. Pollen Electrotransformation for Gene Transfer in Plants / James A. Saunders, Benjamin F. Matthews, Sally L. Van Wert 227

16. Electrofusion of Plant Protoplasts and the Production of Somatic Hybrids / George W. Bates 249

17. Electrotransformation of Bacteria by Plasmid DNA / Jack T. Trevors, Bruce M. Chassy, William J. Dower, Hans P. Blaschek 265

18. Creating Vast Peptide Expression Libraries: Electroporation as a Tool to Construct Plasmid Libraries of Greater than 109 Recombinants / William J. Dower, Steven E. Cwirla 291

19. Electroporation and Electrofusion Using a Pulsed Radio-Frequency Electric Field / Donald C. Chang, John R. Hunt, Qiang Zheng, Pie-Qiang Gao 303

20. Electroinsertion: An Electrical Method for Protein Implantation into Cell Membranes / Youssef Mouneimne, Pierre-Francois Tosi, Roula Barhoumi, Claude Nicolau 327

21. Electroporation as a Tool to Study Enzyme Activities in Situ / R. R. Swezey, D. Epel 347

22. Comparison of PEG-Induced and Electric Field-Mediated Cell Fusion in the Generation of Monoclonal Antibodies against a Variety of Soluble and Cellular Antigens / Uwe Karsten, Peter Stolley, Bertolt Seidel 363

23. Production of Genetically Identical Embryos by Electrofusion / Lawrence Charles Smith 371

24. Development of Cell-Tissue Electrofusion for Biological Applications / Richard Heller, Richard Gilbert 393

25. Novel Applications of Electroporation / Sean R. Gallagher, Donald C. Chang 411

Part III Practical Protocols for Electroporation and Electrofusion

26. Design of Protocols for Electroporation and Electrofusion: Selection of Electrical Parameters / Donald C. Chang 429

27. Protocols for Using Electroporation to Stably or Transiently Transfect Mammalian Cells / Huntington Potter 457

28. Optimization of Electroporation Using Reporter Genes / Grant R. MacGregor 465

29. Genetic Manipulation of Plant Cells by Means of Electroporation and Electrofusion / James A. Saunders, George W. Bates 471

30. Protocols for the Transformation of Bacteria by Electroporation / William J. Dower, Bruce M. Chassy, Jack T. Trevors, Hans P. Blaschek 485

31. Protocol for High-Efficiency Yeast Transformation / Daniel M. Becker, Leonard Guarente 501

32. Protocols of Electroporation and Electrofusion for Producing Human Hybridomas / Martin I. Mally, Michael E. McKnight, Mark C. Glassy 507

33. Human Hybridoma Formation by Hypo-Osmolar Electrofusion / S. M. Maseehur Rehman, Susan Perkins, Ulrich Zimmermann, Steven K. H. Foung 523

34. Electrically Induced Fusion and Activation in Nuclear Transplant Embryos / James M. Robl, Philippe Collas, Rafael Fissore, John Dobrinsky 535

Part IV Instrumentation for Electroporation and Electrofusion

35. Pulse Generators for Electrofusion and Electroporation / Bruce M. Chassy, James A. Saunders, Arthur E. Sowers 555.

Notes:

Includes bibliographical references.

ISBN:

0121680401

012168041X

OCLC:

23868123