Guide to yeast genetics and molecular biology / edited by Christine Guthrie, Gerald R. Fink.

Format:

Book

Contributor:

Guthrie, Christine.

Fink, Gerald R.

Series:

Methods in enzymology ; v. 194.

Methods in enzymology ; . 194

Language:

English

Subjects (All):

Yeast fungi--Genetics.

Yeast fungi.

Yeast fungi--Cytology.

Physical Description:

xxxvii, 933 pages : illustrations ;c24 cm.

Place of Publication:

San Diego : Academic Press, Inc., 1991.

Summary:

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made "Saccharomyces cerevisiae" such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* High-efficiency transformation* Preparation of yeast artificial chromosome vectors* Basic Methods of Cell Biology* Immunomicroscopy* Protein targeting assays* Biochemistry of Gene Expression* Vectors for regulated expression* Isolation of labeled and unlabeled DNA, RNA, and protein

Notes:

Includes bibliographical references and indexes.

ISBN:

0121820955

OCLC:

25205978